The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. Integrated stacker option. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical and. Molecular. Plate readers, also known as microplate readers or microplate photometers,. • 0. Gemini EM and SpectraMax M2 Microplate Readers By Yan Zhang, Molecular Devices Corporation, 1311 Orleans Dr. The wavelength and scanning ranges are from 340–850 nm in 1 nm increments. The Absorbance 96 is a uniquely designed microplate reader for assays in 96-well format. Equipped with scanning, shaking and kinetics modules. Plate Formats: 6 to 384-well microplates. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M4 Microplate Reader transforms each well in a microplate to a fixed. 4 Pages. 2 2601A ValitaTiter. The EMax Plus Microplate Reader measures 96-well plates and comes with eight standard filter modes to cover the entire visible range, for applications such as protein quantification, cell viability, cell proliferation and ELISA. Infinite 200 Pro Microplate Reader. System setup and calibration are controlled from within SoftMax Pro Software. The approximate dead volumes are: 96-well plates: V-bottom: 20-30 µl. Devices® SPECTRAmax 190 microplate reader at a wavelength of 600 nm. Published Dec 16, 2020 | Updated Dec 16, 2020. We would like to show you a description here but the site won’t allow us. #ABS PLUS) Methods. Published Dec 16, 2020 | Updated Dec 16, 2020. The screw remains inside the instrument. Highly customizable low volume applications Molecular Devices unique SpectraDrop Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. 5°C at 37°C. Description. 0 fmol/well FITC 200 µL in 96 wells. The EMax® Plus Microplate Reader delivers a robust solution in an entry level platform. 1 OD at 230 nm. acquisition and SoftMax Pro SoftwareMiniMax Imaging Edition. 96-well microplate: 90 sec; White light: 96-well/ 384 well microplate: <2 min. 96-well flat clear bottom white TC-treated microplates (Corning cat. Step 1: Turn on your microplate reader. Infinite 200 Pro Microplate Reader. SpectraMax Microplate Readers. Temperature Control: Ambient +5C up to 42C. Injectors Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. from Molecular Devices are multi-detection microplate readers with dual-monochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). Use black microplates. A control well was set up to verify. Cells were loaded with 2 µM Fluo-4 for one hour. Final BSA Concentration (µg/ml) in assay BSA Volume NanoOrange. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. , Sigma cat. This report demonstrates that the SPECTRAmax GEMINI-XS microplate spectrofluorometer, with its dual scanning monochromator and patented optical sensitivity, can be used to detect a relatively low number of cells expressing GFP. #L6876) • Guanidine hydrochloride (Sigma cat. Wren supernatant was transferred to a fresh 96-well microplate and absorbance recorded at 620 nm using a SpectraMax M3 (Molecular Devices) spectrophotometer. The BioTek 800 TS absorbance reader is an affordable, high-quality microplate reader for assays in 6- to 384-well formats. 19 H mm that meet microplate industry standards. High-throughput screening (HTS) can efficiently assay multiple discrete biological reactions using multi-well microplates. The VersaMax reads 96-well microplates. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. This unlocks the transport slide. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Read the microplate • Make sure the purple plate adapter (if required for your plate reader) is in the microplate reader drawer. ValitaTiter 96-well (Pack of 20). Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. By far the most compact reader on the market, the footprint of the reader is almost as small as the plate itself. ValitaTiter Plus 96-well (1 plate) VAL004. Look for a washer which can handle a variety of plate types, including deep well plates and 96-, 384- and 1536-well plates. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more. : 2 Oct 12 Page 1 of 20 Setup Guide on the Molecular Devices SpectraMax® Paradigm® Microplate Detection Platform Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). is required for optimum performance with standard 96-well and 384-well format microplates for all top-read applications. This unit offers a wavelength range of 340 nm to 850 nm and a bandwidth of 5 nm. Instrument settings can beAbsorbance Detection Cartridges combine scanning and broad spectrum wavelength range to address a variety of nucleic acids, proteins, ELISAs and immunoassays found in the laboratory. F3/F5 Multi-Mode Microplate ReadersBulk Kit (10-plate size, Molecular Devices P/N R8347) HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax iD3 Multi-Mode Microplate Reader; Methods Cell treatment. The Molecular Devices Versamax Microplate Reader INCLUDED:. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. A FlexStation 3 Microplate reader ( Molecular Devices; San Jose, USA) was used to take fluorescence readings every 2 s (excitation 485. SpectraMax 250 User. Powered by a new, optional high-speed EMCCD camera and the new Peak Pro 2 software module, the new FLIPR® Penta High-Throughput Cellular Screening System allows you to measure and analyze peaks of human-derived induced pluripotent stem cells (hiPSCs),. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. tools to expand the boundaries of their research. In May 2011, Molecular Devices SpectraMax M5e reader, chosen for its durability, became the first microplate reader . Both readers were heated to 37ºC prior to setting up . 0 500 1000 1500 2000 2500 3000 3500 time (sec. Technical specifications. For more sample throughput, the system can well. changes in OD 600 were measured by periodically transferring 100-µL aliquots of each bacterial culture to a single well of a 96-well microplate and measuring absorbance at 600 nm using the SpectraMax iD3 reader and SoftMax Pro Software. SpectraMax iD5 Multi-Mode Microplate Reader (Molecular Devices cat. None for any other plate (6, 12, 24, 48 well plates) Flex3: Both Top read and Bottom read. A dependable washer is quiet, easy to clean and leaves no residue, preventing contamination and reducing background. NOTE: Limited support and literature is available for the SpectraMax 250 due to the senescence of the product. ThermoMax performs automatic mixing before and between readings to ensure reliable and precise. Running both standard spectrophotometer and microplate. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time. Click on the crosshair and move it so that it is centered over the well image. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Keywords: fluorescence polarization, trf, fret, igg. Insert the filled microplate into the drawer, matching well A1 with position A1. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. #ID3-STD) L-Tryptophan (Sigma cat. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. Model:. F3/F5 Multi-Mode Microplate Readers. #I2511)Introduction. Microplate readers read biological, chemical or physical events that occur on a microtiter plate. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Standards, controls and blanks were pipetted into the wells and incubated followed by washing with the Molecular Devices MultiWash™+ Microplate Washer. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. 2 mm x 86 mm) Height:. According to Biocompare, “one of the most overlooked pieces of equipment in any lab is the microplate reader. High tolerance for contaminants. This reader utilizes Molecular Devices' patented PathCheck® Sensor to measure the depth (optical pathlength) of samples in a microplate. Suggested plates are black walled. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The plate reader optics are tunable for. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Ensure ongoing compliance of your Molecular Devices microplate readers and be audit ready with comprehensive . Fluorescence Microplate Reader Comparison Chart. 1 FP • Fluorescence Polarization detection for fluorescein- or . #iD5-STD) Methods ATP standard curve. The OptiMax by Molecular Devices is a tunable microplate reader. This can increase throughput and efficiency, thus saving time and space in the lab. 2 Pages. University of Reading Simon Lydford: Molecular Devices (UK) Ltd. 1. With various on-board features and. The reader includes multiple licenses to the SoftMax® Pro software for endpoint and kinetic analysis of microplate. fluorescence microplate reader from Molecular Devices. Bulk Kit (10-plate size): Molecular Devices P/N R8341; HeLa cells (ATCC P/N CCL-2) Staurosporine (Sigma P/N S5921) 96-well black, clear-bottom microplates (Corning P/N 3904) SpectraMax fluorescence microplate reader; Methods. The direct transfer capability reduces reagent consumption and allows for more assay conditions to be explored in a single microplate, making the system more amenable to agonist and antagonist assay formats. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. Programmable liquid handling allows for perfectly timed, automated biochemical and cell-based assays. Outer wells are aligned for proper viewing with FLIPR. #655075 and #781075) SpectraMax i3x Multi-Mode Microplate Reader; SpectraMax Injector Cartridge *Figure 1. 2 OD at 200 nm; this decreased to 0. 2 OD at 200 nm. 00 Add to cart; Molecular Devices EMax Microplate Reader $ 1,295. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. Unparalleled performance on a personalized platform. 300 µL of Detergent Compatible Bradford reagent was added to each well. The accurate positioning of 96-arrayed colonies on rectangular agar plates (in a 96-well scheme) was used to measure each colony with the optics of the plate reader. Setup Guide. Transfer of an assay to 96-well microplate technology increases throughput significantly. Quantitate proteins rapidly with a 5-minute, room-temperature incubation. SpectraMax Paradigm Microplate Reader is a versatile and customizable device that can perform a wide range of detection modes, such as absorbance, fluorescence, luminescence, time-resolved fluorescence, and AlphaScreen. best-in-class data . Paradigm: Delta mP. TempPlate semi-skirted 96-well PCR plate (USA Scientific cat. 46Click the Read button in the SoftMax Proplated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours. Microplate Readers. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. SmartReader 96: Designed for optimal ELISA Testing. The SpectraMax® i3x reader allows you to unravel the mysteries of science by exploring cellular pathways and protein activation and expression in one systemComputer Integration Each Molecular Devices microplate reader is shipped with a license key for the SoftMax® Pro Data Acquisition and Analysis Software that you install on the computer that you use to operate the instrument. # 0310-5217T) 384-well cell wash head (Molecular Devices Cat. #3610) BrightMax sealing films (Genesee cat. Read More. The EMax Plus reader measures flat and round 96-well microplates. 020 fmol 0. #W50-100) SpectraMax® ABS Plus Microplate Reader. While "plate reader" usually refers to the devices described. Flexibility to read a variety of plate formats. Optimal volumes are usually stated as 'working volume', which is 25 - 90 mL. Expt#3 . As a result, assay flexibility is consequence-free. Figure 2-23: Bottle holder To install the bottle holder: 1. A 1:10 serial dilution of ATP was prepared in medium. The SPECTRAmax™ PLUS microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Fastest Read Time - 96-well plate: 20 sec; 384-well plate: 30 sec. Fluorescence Microplate Reader Comparison Chart. Workflows of the MTT Cell Proliferation and Cell Cytotoxicity Assay. 00. Deep well blocks available in 24, 96 and 384-well formats Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. explore more. For FlexStation I/II/3 plate readers, what are the dead-volumes for the compound plate? The FlexStation plate readers assume a 1 mm pipette height from the bottom of the well when aspirating from a compound source plate. ” Promega Corp. This is equivalent to having 511 built-in filters, Polypropylene Deep well Plates are used as small test tubes because of easier sealing, flat or conical shape, square and round well formats with lids and cap. With optimized reagents, option to operate the. The reader can measure flat and round 96-well microplates and ensure accuracy by automatic lamp calibration before each reading. Wavelength Range 200 to 999 nm. Resipher 32x Starter Kit for use with 96-well plates Includes: (1) Resipher Data Hub, (1) Resipher 32x Reader, (3) sterile sensing lids, all cables Turns a 96-well plate into a precise dynamic oxygen consumption reader, measures 32 wells Utilizes Lucid Lab Cloud software for connection to any…. (a) HCT116 cells expressing hyBRET-ERK were plated in 96-well plates and treated with serially diluted. 54: 1. 340‚ 850 nm. All stand-alone instrument functions can be controlled using the software. g. 7°C and the bottom to 36. Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. Software Validation. Optical System: High energy xenon flash lamp. for ValitaTiter Plus standards read on Molecular Devices readers. Triplicates of each BSA concentration were made for this assay plate. ’s GloMax®-96, for instance, is a dedicated luminometer, whereas Bio-Rad Laboratories’ iMark and xMark strictly measure absorbance. The Vmax Kinetic Microplate Reader is designed to measure any of the visual wavelengths so that you can use your substrate of choice. The reduced cost of the device represents the major technical contribution of this manuscript, as costs were reduced 7 fold from previous reports. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set ofMulti-mode microplate readers with fluorescence detection. 6- to 1536-well plates 4 96- to 384-well plates 4 With up to five modes of detection, F3/F5 Multi-Mode Microplate Readers support a wide variety of applications in life science research. Annual recertification of your validation plates ensure that they meet specifications and give you confidence in the performance of your instrument. By measuring optical pathlength, it eliminates the last major distinction between microplate readers and spectrophotometers. Plate Formats: 6 to 384-well microplates. > Molecular Devices LLC > microplate reader. The SpectraMax ABS Plus Microplate Reader can run both cuvette-based and microplate based reader applications on the same instrument. FlexStation 3 Multi-Mode Microplate Reader A five-mode microplate reader with integrated fluid transfer Click “Next”, and the reader will start scanning the four corner wells; a progress indicator will appear: fter the reader finishes scanning the four corner wells, you will see a screen with an image of the upper left corner A well and a pink crosshair. “We continue to expand on our high-quality line of microplate reader platforms with a new line of absorbance microplate readers”, said Greg Milosevich, President of Molecular Devices. 2 mm x 86 mm) Height: between 13. In combination with the SpectraMax Mini Multi-Mode Microplate Reader, it enables increased throughput and detection of DNA as low as 2 ng/μL using just a few microliters of sample. VANTAstar™ Flexible Multi-mode Microplate Reader. MAXline Microplate Readers User’s Manual iii Molecular Devices Corporation Instrument Warranty Molecular Devices Corporation warrants this product against defects in material or workmanship as follows: 1) All parts of the MAXline Microplate Reader excluding the lamp assembly are warranted for aThe Molecular Devices Aquamax 4000 With 96 Well Head AQUAMAX 4096-G is a quality addition to the Molecular Devices lineup. 50 µL of. 1 OD at 230 nm. For fluorescence intensity assays, users can select from top- or bottom-read modes for improved sensitivity in solution and cell-based assays. #G7294). UV/Vis spectrophotometer & microplate reader with built-in cuvette port. Only Molecular Devices microplate plate readers offer the capability to measure the depth (optical. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. This chapter reviews key criteria for microplate selection, including: well number, well volume and shape,. MultiWash+ Microplate Washer, A 96- & 384-well Automated Washer| Molecular Devices Author: Molecular Devices, LLCA polyclonal antibody specific to mouse/rat IL-22 was pre-coated onto a 96-well microplate. Figure 2. 1 pM. GxP regulated industry assessments of microplate readers. Absorbance was read at 480 nm on the SpectraMax ABS Plus reader. We would like to show you a description here but the site won’t allow us. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. At 5 seconds to read a 96-well endpoint assay or a 5 second minimum interval between kinetic reads the VMax Microplate Reader has both speed and performance. Both readers were heated to 37ºC prior to setting up the assay. 2. Upgradeable modules are also available including western blot, cell imaging, and fast kinetics with injectors. Use preconfigured wash, soak, and aspiration protocols or create your own multi-step protocol using the simple touchscreen interface. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Pipet 100-200 µL of deionized water into each well of a clean 96-well microplate. Measure more accurately. Top or bottom detection is available with a simple click of a button. 92: 88. Description. A 96-well microplate reader for absorption spectroscopy was designed, constructed, and tested at a total cost of ca. . Technical specifications Performance specifications Cross-talk < 3 x 10-5 (96-well) Read modesa solid, white, flat-bottom 96-well plate. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. 96-well and 384-well plates; Wavelength(s): 405, 450, 490, 560 and 600nm (Abs). gemini em reader, molecular devices stakmax microplate stacker, softmax pro data analysis, spectrofluorometer, monochromator microplate reader, cell migration assays, cell viability assays, gfp, picogreen dna. It also allows for the elimination of standard curves. The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. Specifications for the SpectraMax L microplate reader. The clear UltraVision FC 96-well microplate (FC_UltraVision (clear, 96)) showed the best performance of the tested microplates, with absorbance of 0. Applications include ELISA, protein quantification, enzyme kinetics and cell-based assays. The FlexStation® 3 Multi-Mode Microplate Reader can be used to run the SpectraMax DuoLuc assay in 96- and 384-well formats with high sensitivity and throughput. The most common microplate format used in academic research laboratories or clinical diagnostic laboratories is 96-well (8 by 12 matrix) with a typical reaction volume between 100 and 200 µL per well. 012 fmol 0. . Plate reader instructions Computer. 14: Average %CV. 2. Align the two slots on the bottle holder with the two pegs on the rail. The SpectraDrop Micro-Volume Microplate offers the ability to use as little as 2˜µL samples with 24- or 64-well plates. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices set of Multi-mode microplate readers with fluorescence detection. Multi-Mode Reader with the StakMax Microplate Handling System from Molecular Devices. 7°C (gradient heating design). The SpectraMax ABS. Molecular Devices' microplate readers. Molecular Devices SpectraMax 384 Plus Microplate Reader. The instruments offer 96- and 384-well microplate-to-microplate fluid transfer (8 or 16 wells at a time), and can read microplates having 6, 12, 24, 48, 96, and 384-well formats. “The ABS Plus reader comes standard with our patented PathCheck® Technology which enables consistent results across a 96- or 384-well plate. Detection by Low noise PMT with simultaneous digital and analog modes. When reading at wavelengths below 340 nm, special UV-transparent, disposable or quartz microplates allowing transmission of the deepEight filters enable visible light absorption measurement applications such as protein quantification, cell viability and ELISA. ZERO BIAS. AquaMax 4000 Microplate Washer (Molecular Devices Cat. Plate readers, also known as microplate readers or microplate photometers, are instruments which are used to detect biological, chemical or physical events of samples in microtiter plates. However, an upgradeable multi-mode reader which includes absorbance, fluorescence and luminescence can address all. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all leading partner robots. The procedure enables direct translation of any published equation. Learn more 0200-7008 6- to 1536-well plates. For more sample throughput, both readers can be easily integrated into full roboticVisionMate 96 (Vial Rack Bottom Label 2D Reader) Ziath: Datapaq Express (Vial Rack Bottom Label 2D Reader). The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Detergent Compatible Bradford assay. Since then, the microplate measurements of DNA, RNA, and proteins that this enabled have become very popular. and reliable microplate stacking solution for Molecular Devices microplate readers and washers. the appropriate well of the microplate. The concentration of total free thiols in the protein. 1 sec. " A versatile UV/VIS spectrophotometer for both 96- and 384-well microplates as well as cuvette. HeLa cells were plated at 20,000 cells per well in 100 µL per well in a black, clear-bottom microplate. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution designed to expand the boundaries of research capabilities. 48 W x 12. ©2017 Molecular Devices, LLC 3/17 0120-1441I Printed in USA Figure 3. All standard 96- and 384-well microplates, strip wells, and filter-bottom micro-plates can be used in the SPECTRAmax PLUS 384. Five-second read per plate. Vrla used the device to quantify the concentration of a small molecule produced by bacteria. Three low volume 24-well bottom slides. SpectraMax 190 and VersaMax readers can accommodate standard 96-well and strip-well microplates. 3. Expanded read mode capabilities Equipped for high performance, the SpectraMax iD5 is HTRF®-certified, DLReady, and SpectraMax® DuoLuc™certified. Photometric and kinetic analysis mode. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. HPV Serology Laboratory Standard Operating Procedure . Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The microplate reader then generates data following a preconfigured protocol that performs the necessary calculations on the data, delivering consistent results. Read absorbance on the SpectraMax microplate reader at the wavelength to be used for your assay, and average the resulting OD values for all wells to obtain the Plate. Instrument design, user interface capabilities, and cost play key roles in purchasing a suitable microplate reader. No additional manuals are available. CHO-M1 cells were plated at a density of 50,000 cells per well (96-well) or 12,500 cells per well (384-well) the day prior to assay. Important things to consider when purchasing a multimode microplate reader. both cuvette-based and microplate reader applications SpectraMax Mini Multi-Mode Microplate Reader. luminescence for a 96-well plate and 40 msec integration time for a 384-well plate:The EMax® Endpoint ELISA Microplate Reader from Molecular Devices, one of the pioneers in microplate detection, offers a ruggedly simple approach to dedicated endpoint assays. 3 OD at 200 nm. Plate Reader with Microplate Adapter, Molecular Devices SpectraMax M series Multi-mode 6. the SpectraMax i3x Multi-Mode Microplate Reader, may also be used for this application. Monochromatic filter is equipped. Supplier: Molecular Devices Next generation SpectraMax® absorbance microplate readers. (12 and 96 well) all: FMax: all: LMax: all: Macro 96 Well Cell Harvester: all: Micro 96 Well Cell Harvester: all: Mini 96 Well Cell HarvesterSpectraMax L Microplate Reader A flexible, sensitive luminometer for 96- and 384-well microplates Key Features • Unsurpassed sensitivity • Low background and cross-talk • Multi-channel configurations for a 6-fold. Molecular Devices gmbH Tel. Place the microplate in the drawer. absorbance, fluorescence, and luminescence with the added functionality of. The contents of the SpectraDrop Microplate kit depend on kit type. /well integration in 96-well luminescence mode and 0. The color touch screen provides a visual user interface, making programming fast and intuitive. SpectraMax i3x Microplate Reader, Molecular Devices (Cat# i3X) 96-well white walled clear bottom plates, Costar (Cat# 3903). #W50-100). The validation kit included individual tubes of diluted fluorophores:. 1700 Online: Sales Offices. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. With various on-board features and capabilities, the ThermoMax is ideal for use in modern laboratories involved in many applications. #ID3-STD) • L-Tryptophan (Sigma cat. Fluorescence Microplate Reader Comparison Chart. Microplate is validated by molecular devices for the FLIPR reader. Our featured SpectraMax® L Microplate Reader Sensitive luminometer with programmable injector options for 96- and 384-well microplates. (FP) configured microplate readers. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax M5e Microplate Reader transforms each well in a microplate to a fixed. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. Shaking: Linear and Orbital. 0 H x 60. 5 mm and 15. We would like to show you a description here but the site won’t allow us. firefly luciferase in 96- and 384-well top read Dynamic range: > 6 decades Cross-talk: < 0. is required for optimum performa nce with standard 96-well and 384-well format microplates for all top-read applications. Microplate method: Pipet 150 µL aliquots of each sample, standard and blank into the designated wells of the microplate. DNA concentration (ng/mL) Average RFU. #T-0254) • Lysozyme (Sigma cat. in space. Solid black plates usually give the least fluorescent background noise, allowing better sensitivity. SpectraMax® Microplate Reader Suite of Products Knowledge Base. 24, 48, 96, 384 Well Plates; Wavelength(s): 250 - 850 nm; Specifications/Features: Shaking, Temperature control, Cuvette port; Read More. Limit of Detection: < 20 attomol ATP per well Dynamic range > 9 orders of magnitude. The fluorescence intensity was captured with a SpectraMax Paradigm Multi-Mode Microplate Reader (Molecular Devices, LLC, San Jose, CA, USA) at 588 nm excitation and 633. for ValitaTiter Plus standards read on Molecular Devices readers. It also integrates with the. EMax Plus Microplate Reader, Molecular Devices,96 Well Plate, LLC (CAT#: STEM-LE-0510-LC) Cytation™ 1 Cell Imaging Multi-Mode Reader, Wavelength Range: 200 to 700 nm, BioTek Instruments (CAT#: STEM-MR-0003-LKN) Perkin Elmer EnVision 2105-0020 Multilabel Microplate Reader, Perkin Elmer (CAT#: STEM-S-0723-LGZ)The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility offered by monochromator-based systems. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax Pro® 7 Software, the SpectraMax® iD3. Retrieving. Please refer to the Obsolete Microplate Reader Models for more information. 384 well polystyrene microplates have plate dimensions of 127. M3-M5e_manual. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. 5. 4% in white 96- and 384-well microplates Sensitivity (ATP-Glow) Optimized 96 wells 3 pM 384 wells 6 pM * With 6 flashes in absorbance and 3 flashes in fluorescence mode and 0. #VAL004 or VAL014) 96-well assay plate • Human IgG for use as standard (e. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of shaking. Danaher Inc 96 well microplate reader 96 Well Microplate Reader, supplied by Danaher Inc, used in various techniques. #PLUS 384) Methods. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). #25-340) Pyrogen-free Dilution Tubes (Lonza cat. 1 pM 1536-well: 0. On the FlexStation 3 reader, the entire series of reactions is monitored in real time with simultaneous on-board reagent pipetting and luminescence detection (Figure 1). Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다.